Fig. 2

Double immunofluorescence staining of Schwann cell cytoplasmic inclusions. Co-localization of phosphorylated α-synuclein (p-α-Syn) and S-100 (a–c), but not TPPP/p25α (d–f) or phosphorylated neurofilament (p-NF) (g–i), in the inclusions. p-α-Syn and ubiquitin (UBQ) are also co-localized in the inclusions (j–l). P-α-Syn (a, d, g, j) appears red, S-100 (b), TPPP/p25α (e), p-NF (h) and UBQ (k) appear green, and overlap of S-100 or UBQ and p-α-Syn (c, l) appears yellow. Bars = 10 μm