MB treatment reduces detergent insoluble TauΔK and mouse Tau. Sarcosyl-extraction of soluble and insoluble Tau from cortex tissue. (a) Protein levels of soluble and insoluble Tau species in untreated TauΔK mice compared to TauΔK mice receiving MB for 14.5mo, 6mo and 3mo detected by the pan-Tau antibody K9JA and the human Tau specific antibody TauY9. MB administration reduces levels of detergent-insoluble human and mouse Tau. (b) Quantification of soluble Tau levels in cortex lysates shows a minor but non-significant decrease of soluble Tau species upon MB treatment. (c) Quantification of insoluble Tau levels in cortex lysates demonstrates a clear decrease of insoluble human and mouse Tau in MB-treated mice. Bars represent mean protein densities (%) ± SEM; statistics: one-way analysis of variances with post-hoc Newman-Keuls multiple comparisons test. Asterisks indicate significant differences in comparison to untreated TauΔK mice; **: p < 0.01; Ab: antibody; mTau: mouse Tau; n, number of samples.