Figure 4

MB treatment reduces detergent insoluble Tau^ΔK and mouse Tau. Sarcosyl-extraction of soluble and insoluble Tau from cortex tissue. (a) Protein levels of soluble and insoluble Tau species in untreated Tau^ΔK mice compared to Tau^ΔK mice receiving MB for 14.5mo, 6mo and 3mo detected by the pan-Tau antibody K9JA and the human Tau specific antibody TauY9. MB administration reduces levels of detergent-insoluble human and mouse Tau. (b) Quantification of soluble Tau levels in cortex lysates shows a minor but non-significant decrease of soluble Tau species upon MB treatment. (c) Quantification of insoluble Tau levels in cortex lysates demonstrates a clear decrease of insoluble human and mouse Tau in MB-treated mice. Bars represent mean protein densities (%) ± SEM; statistics: one-way analysis of variances with post-hoc Newman-Keuls multiple comparisons test. Asterisks indicate significant differences in comparison to untreated Tau^ΔK mice; **: p < 0.01; Ab: antibody; mTau: mouse Tau; n, number of samples.