RNA expression analysis of FUS/TLS KO mice. (a) Genes up- or down-regulated in the striatum and spinal cord of TLS KO mice (8 weeks old) detected using ExonArray. (b) qPCR analysis of up- and down-regulated genes in the striatum of TLS KO mice at 8 weeks (n = 3). All of these genes were significantly down- or up-regulated in the KO mice (p < 0.05, two tailed t-test). (c) qPCR analysis of FUS/TLS family mRNA in Neuro2A cells depleted of endogenous FUS/TLS using RNAi (n = 4). *P < 0.01, ANOVA followed by Dunnett's test (in comparison with con). Con: untransfected cells. (d) Types of alternative RNA processing differentially regulated in the striatum of TLS KO mice. (e) Examples of cassette exon splicing in WT and KO mice. Alternative exons are indicated by red boxes. (f) Examples of alternative terminal exon splicing. Bar charts show qPCR results using primer sets indicated in the left panels (n = 3). See also Additional file 4: Table S3, online resource.