Figure 4
From: Neuron-to-neuron α-synuclein propagation in vivo is independent of neuronal injury
Neurodegenerative changes caused by hα -syn-AAV prep 1 transduction. (a) Rats received a single injection of vehicle (control animals, n = 3), hα-syn-AAV prep 1 (n = 5) or hα-syn-AAV prep 2 (n = 5) into the vagus nerve. Stereological counts of Nissl-stained DMnX neurons were performed at 12 weeks post treatment on tissue sections from the left (injected side) MO. Values are means ± SEM. *P <0.0001 vs. the control or prep 2 group (one-way ANOVA followed by Tukey’s post hoc test, F2,12 = 73.4). (b) The number of neurons immunoreactive for hα-syn was counted stereologically in the left (AAV-injected side) DMnX of rats injected with either hα-syn-AAV prep 1 or prep 2. Analyses were performed at 6 (bars with pattern, n = 7/group) or 12 weeks (solid bars, n = 5/group) post treatment. Values are means ± SEM. *P <0.005 vs. each of the other three groups (one-way ANOVA followed by Tukey’s post hoc test, F3,23 = 10.82).