NHERF1, moesin, and PTEN localize to ependymal polarized structures. A. Schematic NHERF1 structure shows the N-terminal PDZ domains (1 and 2) and the C-terminal ERM-binding (EB) region with selected ligands. B. Serial sections from an ependymoma case showing localization of NHERF1 and moesin to microlumens and rosettes. Normal ependyma shows apical PM labeling with moesin, similar to NHERF1 but not with NF2 (right panels). C-D. Serial sections from an ependymoma case show the apical PM of canals labeled by NHERF1 and PTEN (arrows) antibodies (C) and NHERF1 microlumens distinct from the PDGFRα punctate or linear perinuclear staining (D).