Figure 2

Survival of medulloblastoma cells harboring alterations in TP53 and CTNNB1 after radiotherapy. A) Clonogenic assay on a panel of TP53 wild-type: D283MED, ONS76, MEB-MED-8A(blue) and TP53 mutant:UW228, Daoy (red) medulloblastoma cell lines. TP53 mutant cells exhibit superior radiation resistance (p < 0.01). B) Transfection of the TP53 wild-type (blue) medulloblastoma cells (ONS76) with R175H dominant-negative TP53 mutation (red) results in increased survival of the cells under the radiation treatment (p < 0.01). C) Transfection of TP53 mut cells (UW228) (blue) with S33Y-CTNNB1 mutation (red) resulted in sensitization of cells to radiation (p < 0.05). D) Transfection of TP53 mutant medulloblastoma cells (UW228) with mutant S33Y-β-catenin construct results in nuclear translocation of β-catenin (bottom panel), no nuclear translocation of β-catenin is observed in the control cells, transfected with pcDNA3 construct (top panel): β-catenin (green), nucleus (red), co-localization of β-catenin to nucleus (yellow).