Figure 4

Inflammatory profile of PGC-1alpha overexpressing astrocytes. PGC-1α⁺ astrocytes expressed less IL-6 (A) and CCL2 (B) mRNA under normal conditions and after 24 hr treatment with TNFα/IFNγ compared to mock-transduced astrocytes. Secretion of IL-6 (C) and CCL2 (D) by astrocytes as measured by ELISA is reduced in PGC-1α⁺ astrocytes. TNFα/IFNγ increased ROS production in mock-transduced astrocytes but not in PGC-1α expressing astrocytes (E). *P < 0.05, **P < 0.01, ***P < 0.001 as determined by students T-test.