Figure 6

FGF1 enhances remyelination in organotypic cerebellar slice cultures. After toxic demyelination, cerebellar slice cultures were allowed to remyelinate for 14 days in the absence or presence of 100 ng/ml FGF1. Myelination was assessed by immunostaining (a, b) and quantitative PCR (c–e). a MBP (red), NFL (green) scale bar = 50 μm b Quantification of MBP⁺/NFL⁺ area ratio in FGF1 treated slices compared to untreated controls. MBP⁺ myelin formation is promoted by FGF1. Students t-test *P = 0.0341. c-e After 14 days RNA was extracted, cDNA obtained and transcript levels of c MBP, d Mag and e Mog were measured by qPCR. One-way ANOVA **P < 0.01; *P < 0.05; All error bars represent SEM from three independent experiments.