Spines per 10 μm | Filopodia | Stubby | Mushroom-like | Thin |
---|
WT Ct | 0.56±0.15 | 2.23±0.72 | 12.05±1.44 | 1.00±0.24 |
WT Nic | 0.93±0.22 | 0.94±0.29ω
| 12.82±1.32 | 1.75±0.37 |
Tg Ct | 1.61±0.35σ
| 1.18±0.34 | 10.96±0.86 | 1.72±0.31 |
Tg Nic | 2.29±0.68σ
| 0.65±0.23ω
| 12.21±2.21 | 2.32±0.44 |
- Number of spines per 10 μm of dendrite length, in neurons from wild type (WT) and TgCHRNA5/A3/B4 (Tg) embryos. Cultures were incubated with medium alone (Ct, Control) or containing nicotine (Nic, 3.25 μm) for 48 h (n = 4-5 cells/culture; 3 - 4 cultures/condition from ≥ 3 experiments). Two-way ANOVA genotype effect σ p ≤ 0.05, treatment effect ω p ≤ 0.05.