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Table 2 Effects of nicotine exposure on dendritic spines in pyramidal neurons from hippocampal primary cultures

From: Hippocampal changes produced by overexpression of the human CHRNA5/A3/B4 gene cluster may underlie cognitive deficits rescued by nicotine in transgenic mice

Spines per 10 μm Filopodia Stubby Mushroom-like Thin
WT Ct 0.56±0.15 2.23±0.72 12.05±1.44 1.00±0.24
WT Nic 0.93±0.22 0.94±0.29ω 12.82±1.32 1.75±0.37
Tg Ct 1.61±0.35σ 1.18±0.34 10.96±0.86 1.72±0.31
Tg Nic 2.29±0.68σ 0.65±0.23ω 12.21±2.21 2.32±0.44
  1. Number of spines per 10 μm of dendrite length, in neurons from wild type (WT) and TgCHRNA5/A3/B4 (Tg) embryos. Cultures were incubated with medium alone (Ct, Control) or containing nicotine (Nic, 3.25 μm) for 48 h (n = 4-5 cells/culture; 3 - 4 cultures/condition from ≥ 3 experiments). Two-way ANOVA genotype effect σ p ≤ 0.05, treatment effect ω p ≤ 0.05.