Figure 3From: Frequent and symmetric deposition of misfolded tau oligomers within presynaptic and postsynaptic terminals in Alzheimer’s disease Different morphologies of human synaptoneurosomes fixed over glass slides. Intact, bipartite synapses appear as snowman-like or elongated/protruded structures under the brightfield, with VGluT1 (presynaptic marker) and MAP2 (postsynaptic marker) immunofluorescence on opposing sides. Isolated hemi-synapses and clusters of synapses are more abundant than bipartite structures. The ratio of bipartite synapse/hemi-synapse/clustered synapse is approximately 5%/50%/45%. Organelles negative for synaptic markers account for about half of the brightfield objects, and these were excluded from our analysis of tau immunofluorescence.Back to article page