Figure 7
The immunoreactivities for the mitochondrial fission marker DRP1 and the outer membrane marker TOM20 are both increased in the MSA-oligodendroglial cytoplasm before the obvious formation of GCIs. a: MFN2-immunoreactivity is observed in the neuronal cytoplasm of both the control (Case 15) and MSA (Case 1), whereas as the immunoreaction is barely detected both in normal and MSA-oligodendroglia. b: DRP1 were expressed in the neuronal cytoplasm of both the control and MSA. DRP1 immunoreactivity is seen in some oligodendroglial cell bodies (arrowheads) in an MSA patient, but not obviously in control. c: Triple-labeling immunofluorescence for TPPP (TPPP-N-mab#2A8G3), pα-syn, and either DRP1 (left two columns) or TOM20 (right two columns). Accumulation of DRP1 and TOM20 can be seen in types 2 (a blue arrow for DRP1; a blue arrowhead for TOM20), 3, 4 and 6 oligodendrocytes. d: Western blotting analysis of TOM20 on subcortical white matter on precentral gyrus in control (Cases 26, 35–37) and MSA (Cases 1, 12–14) patients. There is no significant change of amount of TOM20 between MSA and controls. Scale bar = 20 µm (a, b), 10 µm (c).