Figure 6

TPPP relocates to the cytoplasm with the mitochondrial membrane markers TOM20 and cytochrome C in MSA-oligodendroglia. Double-labeling immunofluorescence of TPPP (TPPP-N-mab#2A8G3), TOM20, and cytochrome C in a control (a-h) (Case 16) and an MSA patient (i-p, Case 1). a-d: In normal oligodendroglia, TOM20 (a), a marker of the outer mitochondrial membrane, is detected as few small granules in the cytoplasm, whereas TPPP immunoreactivity is seen diffusely in the cytoplasm (b). e-h: Similarly, cytochrome C (e), an inner mitochondrial membrane marker, is observed as granules in normal oligodendroglia. i-l: In an MSA-oligodendroglia, TOM20 is accumulated in the swollen perinuclear cytoplasm (i), with a similar pattern with TPPP (l). m-p: In another MSA-oligodendroglia, cytochrome C is accumulated in the large perinuclear cytoplasm (m), again in a similar pattern with TPPP (n). All scale bars = 10 µm.