Figure 2

Double-labeling immunofluorescence studies demonstrating the localization of TPPP throughout the oligodendroglia, including the nucleus. Staining was performed for myelin basic protein (MBP) (a, d), OLIG2 (e, h), GFAP (i, l), and IBA1 (m, p), in the cerebellum of a control case (Case 25). a-d: Most of the MBP immunoreaction (a) appears co-localize with TPPP immunoreaction (b) in the cerebellar folial white matter. e-h: TPPP colocalizes with OLIG2 expressed in the oligodendroglial nucleus. Note that additional TPPP staining that does not colocalize with the OLIG2 staining (f & h, arrowheads), suggesting that TPPP is expressed also in the oligodendrolglial cytoplasm. i-l: TPPP (j) does not colocalize with the astroglial marker GFAP (i). m-p: TPPP (n) does not colocalize with the microglial marker IBA1 (m). q: Three-dimensional images of double-labeling immunofluorescence against TPPP and lamin B1 reveal that TPPP additionally localizes in the nucleus of oligodendroglia. r: Not every oligodendroglia shows nuclear TPPP staining. An arrow indicates an oligodendroglia without nuclear staining, whereas an arrowhead indicates an oligodendroglia with nuclear TPPP staining. a-q: TPPP-N-mab#2A8G3, r: TPPP-C-psB. Scale bar = 200 µm (a-d), 10 µm (e-p), 20 µm (q, r).