Figure 1

Two novel antibodies, TPPP-C-psB and TPPP-N-mab#2A8G3 recognize endogenous TPPP that is specifically expressed in the brain. a: TPPP-C-psB and TPPP-N-mab#2A8G3 both specifically recognize a 25 kDa protein in the mouse brain. These reactions are blocked by absorption tests (peptide (+)). b: Western blots of the homogenates of various organs from an adult mouse demonstrate that TPPP is a brain-specific protein. c & d: Some glial nuclei in the cerebellar white matter as well as perinuclear cytoplasm and neuropil show TPPP-immunoreactivity using TPPP-C-psB (c), while this was not obvious in most cells when formic acid treatment was omitted (d) (Case 25). e-h: Immunohistochemistry using TPPP-C-psB (e & g) and TPPP-N-mab#2A8G3 (f & h) in normal control cerebellum (Case 25). The white matter (e & f) are stained intensely, whereas, granular (* in e and f) and molecular († in e and f) layers in the cortex are not. In addition to neuropil and perinuclear structures, TPPP-immunoreactivity is seen in some glial nuclei (g & h, peptide (–)). These reactions are also blocked by absorption tests (g & h, peptide (+)). Scale bar = 50 µm (c, d), 200 µm (e, f), 20 µm (g, h).