Figure 2
Transversal spinal cord sections (7 μm) from SOD1 G93A transgenic mice at terminal disease state and from control mice. (a) HE-stained section of a SOD1G93A mouse spinal cord. (b) Immunostaining of human SOD1 with PET-blot technique without proteolysis reveals strong staining of the grey matter of a SOD1G93A spinal cord. If the PET-blot is treated with PK labelling of grey matter area is left (c). In the PET-blot of control tissue from a wild type mouse also strong labelling applying an antibody recognizing endogenous SOD1 observed (d) which is completely abolished by limited proteolysis (e). Higher magnification of the PET blots in b and c revealed the pronounced cellular staining in the ventral horn area of the untreated spinal cord PET-blot (f). In the PK treated PET-blot large numbers of aggregates of different size and shape are detected (g). Staining with an antibody against ubiquitin is shown in an overview (h) and at higher magnification (i) for comparison. Bars represent 20 μm (i), 50 μm (g), 100 μm (f), and 200 μm (d, e, h).