Figure 3

Total amyloid burden. Immunohistochemistry (6E10/4G8) showed a notable reduction in total amyloid burden in cortical and hippocampal sections of CpG ODN-treated mice compared with sections of vehicle-treated mice in both study groups ( A - H ). Quantitative stereological analysis of total amyloid burden revealed a significant reduction in 3xTg-AD mice treated with CpG ODN compared with control Tg mice. There was a 67% reduction (**p = 0.018) in total cortical amyloid burden ( I ) and 47% reduction (*p = 0.0032) in total hippocampal amyloid burden ( J ) in the 7–20 month study group. Similarly, there was a 64% reduction (*p = 0.047) in total cortical amyloid burden ( K ) and 60% reduction (**p = 0.0013) in total hippocampal amyloid burden ( L ) in the 11–18 month study group. Scale bars (100 µm) in B and F correspond to cortical images A, B, E, F. Scale bars in D and H correspond to hippocampal images C, D, G, H.