Table 3 Antibodies and conjugates used for double labeling
Antigen | Species | Final dilution | Source | Secondary ab’s and conjugates |
|---|---|---|---|---|
Human Iba1 | Rabbit | 1:2000 | WAKO chemicals | DoaR-AF488 1:400 |
Cow GFAP | Rabbit | 1:2000 | DAKO | DoaR-AF488 1:400 |
Human CD68 | Mouse | 1:300 | DAKO, clone KP1 | DoaM-AF488 1:400 |
Human TLR2 | Goat | 1:500 | R&D systems | DoaG-AF594 1:400 |