Figure 5

Neuronal loss and increased expression of cytotoxic factors in the VPM/VPL of aged PGRN-deficient mice. A, Nissl-stained images of the VPM/VPL of 10- and 90-week-old WT and KO mice (scale bar = 50 μm). B, The number of Nissl-positive cells in the VPM/VPL of 10- and 90-week-old WT and KO mice. The data are presented as the change relative to the number of 10-week-old WT mice. Values are presented as the mean ± SEM (n = 5 for each group). Values having a different superscript are significantly different (P < 0.05, Tukey-Kramer test). C, Comparison of gene expression for Mpeg1, C4, Cyba, Cybb, TNF-α, and Lcn2 between 90-week-old WT and KO mice in the thalamus. The data are presented as the change relative to the level in WT mice. Values are the mean ± SEM (n = 6 for WT; n = 5 for KO, *P < 0.05, unpaired t-test). D, Iba1-immunostained images in the layer IV of the S1BF of 10- and 90-week-old WT and KO mice (scale bar = 100 μm). E, The number of Iba1-IR cells in the VPM/VPL of 10- and 90-week-old WT and KO mice. The data are presented as the change relative to the number of 10-week-old WT mice. Values are presented as the mean ± SEM (n = 5 for each group). Values having a different superscript are significantly different (P < 0.05, Tukey-Kramer test).