Table 1 Antibodies used for IHC and FACS
Immunohistochemistry | |||
|---|---|---|---|
| Â | Company | Cat # | Dilution |
Antibody (primary) | |||
PLP1 (clone plpc1) | AbD Serotec | MCA839G | 1:500 |
HLA-DR (clone LN3) | eBioscience | 14-9956 | 1:750 |
FCGR2B (datasheet CD32B) | Biorbyt | orb44658 | 1:350 |
ASAH1 | Abcam | ab74469 | 1:500 |
LGALS1 | Â | Â | 1:100 |
Claudin5 (clone 4C3C2) | Thermo Fisher Scientific | 35-2500 | 1:250 |
IBA1 | Wako | 019-19,741 | 1:1000 |
IBA1 | Abcam | ab5076 | 1:750 |
Ki67 (Clone B56) | BD Pharmingen | 556,003 | 1:400 |
Antibody (secondary) | |||
Biotin-Donkey anti-Rabbit IgG | JacksonImmunoResearch | 711-065-152 | 1:300 |
Cy3 Goat anti-Mouse IgG | JacksonImmunoResearch | 115-165-003 | 1:200 |
Donkey anti-Mouse, Alexa Fluorâ„¢ 594 | Thermo Fisher Scientific | A21203 | 1:300 |
Donkey anti-Goat, Alexa Fluorâ„¢ 633 | Thermo Fisher Scientific | A21082 | 1:300 |
Streptavidin, Alexa Fluorâ„¢ 488 | Thermo Fisher Scientific | S11223 | 1:300 |
FACS | |||
|---|---|---|---|
Antibody (primary) | Company | Cat # | Dilution |
FITC anti-human CD45 | BioLegend | 304,006 | 1:25 |
PE anti-human CD11b | BioLegend | 301,306 | 1:40 |