Figure 3
From: Loss of the neuroprotective factor Sphingosine 1-phosphate early in Alzheimer’s disease pathogenesis
Reduced SphK1 activity in the hippocampus of Braak III/IV subjects. SphK1 activity in hippocampus (A) and temporal GM (B) tissue extracts of control (n = 9), Braak stage I/II (n = 8), Braak III/IV (n = 7), and Braak V/VI (n = 10) brains. Results shown are the mean and standard error for combined results derived from two (B) or three (A) independent assays. (C) SphK1 protein levels in the hippocampus were determined by western blotting and normalised to housekeeping gene β-actin. (D) Example western blot showing SphK1 protein in hippocampus tissue samples. Braak stage is indicated for each sample. Statistical significance was determined using a one-way ANOVA and Dunnett’s post test.