Figure 2

Cell identification using WSA automated and manual. (A) In some cases, WSA automated quantified NeuN immunopositive cells close to the grey/white matter demarcation (white arrow); some of these cells might be neurons in the cortical layer VI. (B) In contrast, because the user drew the white matter with a set distance away (0.5 mm) from the grey/white matter demarcation in WSA manual, we could be confident that the counted cells were in the white matter.