Figure 5

Lack of C4F6 immunoreactivity in tissue from sALS cases. For a point of reference, tissues from non-transgenic mice (a), WT (b), and L126Z (c) hSOD1 overexpressing mice were co-stained. These tissues were steamed in citrate buffer to enhance C4F6 immunoreactivity as described in Figure 5. (d-f) C4F6 staining of human tissue from non-disease controls revealed occasional spheroid (arrow in panel e) and punctate immunoreactive deposits (3 cases shown). (g, h) Similar structures were observed in human tissue from sALS cases (2 cases shown). For both the control and sALS cases the images shown are from tissues steamed in citrate. Less staining was observed in untreated tissues (Additional file 4: Figure S3). (i) The spheroid deposits were identical in appearance and frequency to structures identified as corpora amylacea (highlighted with arrows) by the Periodic Acid-Schiff stain. Scale bar 100 μm.