Figure 7
Comparison of OCT with histological analysis. A: Representative excerpt of an OCT B-scan through the optic nerve head and a corresponding micrograph of a 0.5 μm thick semi-thin section. Scale bars, 200 μm in OCT scan and 100 μm in micrograph. Thickness measurements of composite layers acquired by OCT correlated significantly with values measured in histological sections (n = 4 per group). Pearson’s correlation. Cln3 +/+, P < 0.01. Cln3 -/-, P < 0.01. Ppt1 +/+, P < 0.001. Ppt1 -/-, P < 0.01. Coefficients of determination (R2) are indicated for the different groups. B: Electron micrographs of retinal layers from 7-month-old Ppt1 -/- and 16-month-old Cln3 +/+ and Cln3 -/- mice. Accumulation of granular osmiophilic deposits (GRODs; arrows) was mostly observed in cells in the GCL and INL of Ppt1 -/- mice, but not in Ppt1 +/+ (not shown) or Cln3 +/+ controls. Similarly, fingerprint profiles (FPs; arrowheads) were predominantly detected in cells in the GCL and INL of Cln3 -/- mice, but not in Ppt1 +/+ or Cln3 +/+ mice. Scale bars: 0.2 μm and 5 μm.