Experimental setup and description of image acquisition settings. A: Additional equipment for analysis of mouse eyes included a polymethylmethacrylate hard contact lens (arrow) and a +25 diopter add-on lens. B: Depiction of the custom-built rodent mount and positioning of the animal to allow imaging centered on the optic disc. C-G: Representative examples of the different imaging modes exemplarily shown for a Ppt1
+/+ mouse. C: infrared fundus image (IR), D: blue laser autofluorescence fundus image (BAF), E: OCT star scan (6 B-scans), F: OCT volume scan (49 B-scans), G: OCT peripapillary circle scan. Field of view, automatic real-time tracking (ART) and resolution settings are indicated in the corresponding images; HR, high resolution. For clarification, 3D-visualizations of OCT scans are depicted. Scale bars correspond to 50 μm of subtended retina in C, D and to 100 μm in fundus images in E, F, G.