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Table 1 List of antibodies used in the study

From: A new animal model of spontaneous autoimmune peripheral polyneuropathy: implications for Guillain-Barré syndrome

Antibody/dye Specificity Immunogen/clone Dilution Source Catalog#
Primary antibodies for IHC      
Rabbit anti ionized calcium-binding adaptor molecula 1 (Iba-1) 17 kDa EF hand protein expressed on macrophages and microglia Synthetic peptide of C-terminus Iba-1 N’-PTGPPAKKAISELP-C’ 1:1000 Wako 019-19741
Rat anti mouse CD86 Mouse B7.2 LPS-activated CBA/Ca mouse splenic B cells 1:600 Biolegend 105010
Rabbit anti protein gene Product 9.5 (PGP9.5) All mammalian species, PGP9.5 expressed on neuronal cell bodies and axons in central and peripheral neural system Human PGP 9.5 Protein purified from pathogen-free human brain 1:800 Ultraclone RA95101
Rabbit anti human activating transcription Factor 3 (ATF3) Mouse, rat and human, ATF3 expressed on damaged neurons A peptide mapping at the C-terminus of human ATF-3 1:2000 Santa cruz SC188
Rat anti mouse CD8 alpha Mouse CD8 alpha Mouse thymus/spleen cells 1:2000 (fresh frozen tissues with short fixation) BD pharmingen 550281
Rabbit anti glucose transporters-1 (Glut1) Mouse, rat and human, Glut1expressed on endothelial cells and perinurium Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV) 1:6000 Merck millipore 07-1401
Secondary antibodies for IHC      
Alexa fluor 594 goat anti-rabbit IgG IgG heavy chains and all classes of IgG light chains from rabbit   1:500 Molecular probes (Invitrogen) A11037
Alexa fluor 488 goat anti-rabbit IgG IgG heavy chains and all classes of IgG light chains from rabbit   1:500 Molecular probes (Invitrogen) A11034
Alexa fluor 594 rabbit anti-rat IgG IgG heavy chains and all classes of IgG light chains from rat   1:500 Molecular probes (Invitrogen) A11007
Alexa fluor 488 rabbit anti-rat IgG IgG heavy chains and all classes of IgG light chains from rat   1:500 Molecular probes (Invitrogen) A11006
Dye      
4′,6-diamidino-2-phenylindole dihydrochloride (DAPI)    1:15,000 Sigma-aldrch D9542
Fluoro myelin red fluorescent myelin stain    1:300 Molecular probes (Invitrogen) F34652
Flow cytometric antibodies      
FITC rat anti-mouse CD11b Mouse CD11b+ cells C57BL/10 splenic T cells and concanavalin A-activated C57BL/10 splenocytes 1:50 BD pharmingen 553310
Rat anti-mouse CD45 APC Mouse CD45+ cells Clone 30‒F11 1:50 eBioscience 17-0451-82
Rat anti-mouse CD8 alpha PerCP-Cy5.5 Mouse CD8+ cells Clone 53-6.7 1:50 eBioscience 45-0081-82
Rat anti-mouse CD86 (B7.2) PE Mouse B7.2+ cells Clone GL1 1:50 eBioscience 12-0862-82