Figure 6

Blood nerve barrier in the sciatic nerves of diseased L31/CD4 ^-/- mice was disrupted. The integrity of neural barriers (BBB, BSCB and BNB) was examined with a fluorescent dye NaFlu (MW, 376 Da) injected intravenously. Quantification of NaFlu content in the brain, the spinal cord and the sciatic nerve of wild type and the symptomatic L31/CD4^-/- mice revealed that a significant increase of the dye within the nervous tissues was detected only in the nerve (n = 3/group, ***: p < 0.001 ) (A). Histological analysis on the cross sections of the sciatic nerves confirmed the dye extravasation in the symptomatic L31 mice. Glut1 was used to label blood vessels, perinurium and epineuriums (B). Scale bar: 100 μm.