Figure 2

L31/CD4 ^-/- mice developed spontaneous motor and sensory neurological disorders. The incidence of motor deficits in L31/CD4^-/- mice evaluated with established clinical scores was presented in (A). Rotarod test was performed to examine coordination ability. Compared with wild type mice, the latency of fall dropped in both pre-symptomatic and symptomatic L31 mice (B) (n = 6/group, **: p < 0.01, ***: p < 0.001), indicative of motor impairment. Pain responses to mechanical, cold and heat stimuli were assessed in wild type, pre-symptomatic and symptomatic L31/CD4^-/- mice. Animals that had their clinical score ≥4 were excluded from sensory tests. Only symptomatic L31/CD4^-/- mice demonstrated numbness to von Frey hair stimulation (C) (n = 6/group, ***: p < 0.001). However, thermal hypersensitivity was observed in both pre-symptomatic and symptomatic L31 mice, as the duration of withdrawal in acetone test increased (D) (n = 6/group, *: p < 0.05, ***: p < 0.001), and the hotplate latency decreased (E) (n = 6/group, **: p < 0.01, ***: p < 0.001).