Figure 4

Electron micrographs showing high white matter ultrastructural quality in frozen cut sections from case 3. (A) Low magnification EM displaying numerous myelinated axons of varying sizes. Note an oligodendrocyte cell body located on left side of images; bar = 2 μm. (B, C) High magnification EM images showing myelinated axons with some subcellular organelles preserved, such as microtubules, neurofilaments and a few mitochondria; bars = 1 μm. (D) Higher magnification EM image showing a myelinated axon with numerous compact myelin sheaths. The inset demonstrates clarity of myelin sheath layers; bar = 0.2μm, bar in inset = 0.1 μm.