Generation of iPSC lines from archived non-cryoprotected biobanked dura mater

Table 1 Successful dural outgrowths and iPSC generation

Sample	CP	Sex	Age	Class.	iPSCs	PMI (Frozen)
ASC2S/D	Y	F	47	MSA	3+ clones	14’24”
ASC7D	N	F	79	AD	3+ clones	7’40”
ASC8D	N	F	54	Control	Contaminated	15’40”
ASC9D	N	F	78	AD	Insufficient #	34’55”
ASC12D	N	M	68	Control	Failed	20’46”
ASC14D	N	F	78	AD	N/A	34’55”
ASC15D	N	F	79	AD	N/A	7’40”
ASC19D	N	M	60	ALS	2+ clones	11’45”
ASC21D	N	M	89	Control	Failed	7’17”
ASC22D	N	F	54	Control	Failed	15’40”
ASC24D	N	M	72	DLBD	Failed	23’55”
ASC27D	N	M	63	HD	2+ clones	14’55”
ASC30D	N	F	76	PD	2+ clones	13’34”

Abbreviations. For sample, S refers to skin as tissue of origin, D refers to dura mater. CP stands for cyroprotection. Class stands for classification, which includes MSA (multiple systems atrophy), AD (Alzheimer’s disease), ALS (amyotrophic lateral sclerosis), DLBD (diffuse Lewy body disease), HD (Huntington’s disease), and PD (Parkinson’s disease). For iPSC generation, N/A (not applicable) indicates no reprogramming was attempted although the number of outgrowth cells was sufficient. PMI refers to post mortem interval, and refers to the amount of time after death before the sample was frozen in hours (’) and minutes (”).

ISSN: 2051-5960