Figure 4
From: Novel monoclonal antibodies to normal and pathologically altered human TDP-43 proteins
Preferential recognition of pathological TDP-43 by MAb 138. MAb 138 robustly detects pathological TDP-43 inclusions (arrows) with no or very minimal nuclear staining in FTLD temporal cortex (a) and little recognition of normal nuclear TDP-43 in human control dentate gyrus (e), similar to previously described antibodies specific for phospho TDP-43 (b, f). In contrast MAb 5060, the epitope for which lies within aa 182–261, and MAb 5195 which detects an epitope contained within aa 394–414, recognize both pathological TDP-43 inclusions (arrows) and normal nuclear TDP-43 (asterisks) in FTLD temporal cortex (c,d) and control dentate gyrus (g,h). Scale bar = 50 μm.