Illustration of a single plaque over the whole imaging period of 15.5 months. (a) Time series of a single plaque as a surface-rendered object as derived from 3D image analysis. Scale bar represents 100 μm. (b) Volume from the plaque shown in (a) over time (open circles) with the corresponding fit of a sigmoid function (black line). (c) Sigmoid fits of plaque volumes of all imaged plaques in one mouse. Note the considerable variance in plaque volumes.