Effects of SRTAW04 on demyelination in the spinal cord. (a) Demyelination, scored on a relative scale by histologic evaluation of LFB stained spinal cords (n = 4-5 per group) taken 30 days after infection, shows a significant increase (***p < 0.001) in the RSA59 infected group compared to control and RSMHV2 infected mice. Treatment with SRTAW04 shows a significant reduction in the demyelinating score (@@@p < 0.001). Treatment group that received SRTAW04 with EX527 showed a significant increase (@@p < 0.01) in demyelinating score compared to the group that received SRTAW04 alone, but no significant change compared to untreated RSA59 infected mice. LFB (left) and neurofilament (right) stained serial spinal cord sections from a representative (b,c) control, (d,e) RSMHV2 infected, (f,g) RSA59 infected, (h,i) RSA59 infected, with SRTAW04 treatment and (j,k) RSA59 infected, with SRTAW04 + EX527 treatment mouse. Neurofilament staining shows axonal loss limited to the areas of demyelination seen on LFB stained sections. Scale bars 500 μm for b-k.