Figure 6
Effects of SRTAW04 on expression of markers of mitochondrial and anti-oxidant function. (a) Western blot of protein extracts from optic nerve and retina of control (lanes 1–4), MHV-A59 infected (lanes 5–8), and MHV-A59 infected + SRTAW04-treated (lanes 9–12) mice. Average levels of SDHb measured by Western blotting (n = 4/group) showed a significant (*p < 0.05) decrease in protein extracts from optic nerves (a,b) and retinas (a,d) of MHV-A59 infected mice 7 days post-inoculation, compared to control mice. MHV-A59 infected mice treated with SRTAW04 (100 mg/kg/day) showed a significant increase (@p < 0.05) of SDHb protein expression compared to untreated MHV-A59 infected mice. There is a significant decrease (*p < 0.05) in expression of SOD2 (n = 4/group) in optic nerves (a,c) and retinas (a,e) of MHV-A59 infected mice compared to control mice, and treatment with SRTAW04 significantly (@p < 0.05) attenuates that change. PGC1-α expression shows a significant (*p < 0.05) decrease in retinas (a,f) (n = 4/group) during MHV-A59 infection and treatment with SRTAW04 for 7 days significantly (@p < 0.05) increases the PGC1-α protein levels.