Figure 5
SRTAW04 treatment reduces ROS in MHV-A59 infected optic nerves both 7 and 30 days after infection. Mice were infected with MHV-A59 and sacrificed 7 days post-inoculation, when optic nerve inflammation is known to peak. (a) Representative images show optic nerves stained with MitoSOX Red. There is an increase in staining of the superoxide anion in MHV-A59 infected optic nerve compared to control optic nerves, with less staining present in optic nerve from an MHV-A59 infected mouse treated with SRTAW04 (100 mg/kg/day) for 7 days. (b) Quantification of MitoSOX Red staining in optic nerves. Average optical density in the central area of optic nerves was determined with image J software. Treatment with SRTAW04 (n = 8) significantly (@p < 0.05) attenuated the increase in superoxide anion staining induced during MHV-A59 (n = 6) infection relative to controls (*p < 0.05). (c) Representative images show optic nerves stained with MitoSOX Red 30 days after infection with MHV-A59. There is increased staining in MHV-A59 infected optic nerve compared to control optic nerve, with less staining present in optic nerve from an MHV-A59 infected mouse treated with SRTAW04 (100 mg/kg/day) for 30 days. (d) Treatment with SRTAW04 (n = 8) significantly (@p < 0.05) attenuated the increase in average optical density of superoxide anion staining observed in MHV-A59 (n = 7) infection relative to controls (n = 5) (*p < 0.05). Scale bars 100 μm for a,c.