Alpha-synuclein spreading in M83 mice brain revealed by detection of pathological α-synuclein by enhanced ELISA

Table 2 Antibodies used in this study

*Antibodies*	Epitopic specificity	*Type*	*Source*	*Dilution (ELISA/WB/IHC)*
LB509 (human α-syn)	115-122	Mouse monoclonal	Abcam (ref ab27766)	1/2000^E -1/1000^WB
4D6 (α-syn)	124–134	Mouse monoclonal	Abcam (ref ab1903)	1/2000^E
clone42 (α-syn)	91–96	Mouse monoclonal	BD Biosciences (ref 610787)	1/2000^E/WB
syn514 (α-syn)	2–12	Mouse monoclonal	Abcam (ref ab24717)	1/500^E
8A5 (α-syn)	129–140	Mouse monoclonal	Provided by Dr. Anderson	1/2000^E
D37A6 (mouse α-syn)	103–110	Rabbit polyclonal	Cell Signaling (ref 4179)	1/1000^E
phosphorylated α-syn*	PSer129	Rabbit polyclonal	Abcam (ref ab59264)	1/3000^E -1/1000^WB
EP1536Y (phosphorylated α-syn)**	PSer129	Rabbit monoclonal	Abcam (ref ab51253)	1/1000^WB-1/300^IHC
EP1537Y (β-syn)		Rabbit monoclonal	Abcam (ref ab76111)	1/5000^WB
Actin		Mouse monoclonal	Abcam (ref ab8226)	1/2000^WB

All antibodies were used in ELISA with polyclonal anti α-syn antibody (Millipore ref AB5038P) as capture, unless mentioned.
*Antibody used in ELISA with monoclonal anti α-syn clone 42 as capture.
**Antibody used in Western blot and immunochemistry.
E: ELISA, WB: Western blot.

ISSN: 2051-5960