Figure 5

Species and time-specific detection of disease-associated α-synuclein ( α S ^D ) in selected neuro-anatomical regions. The following neuro-anatomical regions were sampled from sick M83 mice: Me: mesencephalon, BS: brain stem, SC: spinal cord. A. ELISA results for 4 mice (1–4) inoculated (Inoc) with brain extract from sick M83 mice (P2) 12 weeks after the intra-cerebral inoculation, compared to an age-matched uninoculated mouse (Uninoc). B. Detection of αS^D of mouse origin in sick M83 mice using a mouse specific anti-αS antibody, D37A6, in an uninoculated (Uninoc) sick mouse (276 days old) and in sick M83 mice inoculated with brain extracts from sick mice (P1) or with fibrillar human recombinant αS (231 and 54 days post-inoculation respectively).