CGRP positive axonal loss within the dorsal horn develops with EAE, is reduced following early tolerisation but still progresses even in the absence of further clinical relapses. CGRP positive primary afferents in the dorsal horn in normal, naïve control mice (a, N = 4), as EAE progresses at day 29 (b, N = 5), day 58 (c, N = 5) and day 105-crEAE (d, N = 6), as well as following early (e, N = 4) and late (f, N = 7) tolerisation. Quantification (g) shows a progressive loss (black asterisk) over disease course that is reduced following early tolerisation, however still persists when compared to day 29 (g - red bar, lines and asterisk). Late tolerisation had no significant impact on progression of CGRP loss (g, green bar). Scale bar = 100 μm for all images, significance for all data, P < 0.01, one-way ANOVA.