Figure 8

Immunogold labeling TEM within amyloid plaques from AD brains. a) Shows double labeling using the dityrosine antibody and anti-Aβ antibody on an AD brain section and reveals very dense labeling of the amyloid plaques. This is compared to a serial section b) Showing single labelling using an irrelevant antibody against hair cell antigen (HCA) to show the specificity of the dityrosine labeling on plaques. This image shows virtually no gold labeling. c) Shows single labeling using the dityrosine antibody to reveal very clear labeling at increasing magnification (ci, cii and ciii) showing that the dityrosine labels fibrils in the plaques. d) Double labeling with anti-Aβ (5 nm) and anti-dityrosine (10 nm) confirms colocalisation of dityrosine with Aβ within amyloid plaques at increasing magnifications (di and dii). e) Shows the 10 nm labeling for dityrosine highlighted in red and 5 nm labeling for Aβ in blue. Inserts show magnified images for further clarity. f) and g) show additional examples of co-immunogold labeling in brain from additional patients.