Figure 4

Co-localisation studies of abnormal PrP (Alexa 546, red) with Neurofilament (A, C, E, G) or with myelin basic protein (B, D, F, H), (green, Alexa 488) in the C-terminal E200K mutation and in sCJD control cases (frontal cortex). A, B, E200K mutations shows rare filamentous PrP, co-localising with the myelin sheath (B, B’) but not detectably with axonal neurofilament (A, A’). All sCJD cases (129VV, C, D; 129MV, E, F; 129MM, G, H) show variable amounts of granular PrP which does not co-localise with neurofilaments (C, E, G) or Myelin basic protein (D, F, H). Scale bar: 16 μm for A-H and 4 μm respectively for A’-H’.