Figure 4
Modelling of MV2K. (a) Immunohistochemical analysis of PrP in the brains of VV2-inoculated PrP-humanized mice. G, grey matter; W, white matter. Scale bar: 100 μm. (b) Summary of the distribution of PrP plaques in the brains of VV2-inoculated mice. The distribution of PrP plaques (black dots) was plotted on the illustrations showing the level of bregma −1.79 mm from “The Mouse Brain, 4th edition” [30]. (c) Western blot analysis of PrPSc in the brains using the anti-PrP antibody 3F4 and the PrPSc-type specific antibodies T1 or T2. (d) The mean signal intensities of 3F4-, T1-, or T2-reactive PrPSc. To compare the amounts of the T1- or T2-reactive PrPSc among the mice, the signal intensities of the T1- or T2-reactive PrPSc were normalized by those of the 3F4-reactive PrPSc. The mean signal intensities of MM1-inoculated Ki-Hu129M/M were assigned as 100 /mm2 in each experiment using the 3F4 or T1 antibody, and those of VV2-inoculated Ki-Hu129V/V were assigned as 100 /mm2 in each experiment using the T2 antibody. All experiments were repeated independently at least three times. (e) Western blot analysis of deglycosylated PrPSc using the 3F4 antibody. Ki-Hu129M/M produced the intermediate type PrPSc located at 20 kDa, Ki-Hu129V/V produced type 2 PrPSc located at 19 kDa, and Ki-Hu129M/V produced a mixture of these PrPSc bands.