Transmission properties of MV2K and MV2K + C. (a) Immunohistochemical analysis of PrP in the brains of MV2K- or MV2K + C-inoculated mice. Ki-Hu129M/V showed fewer PrP plaques (arrows) compared with Ki-Hu129M/M. G, grey matter; W, white matter. Scale bar: 100 μm. (b) Summary of the distribution of PrP plaques in the brains of MV2K- or MV2K + C-inoculated mice. The distribution of PrP plaques (black dots) was plotted on the illustrations showing the level of bregma −1.79 mm from “The Mouse Brain, 4th edition” . (c) Western blot analysis of PrPSc in the brains using anti-PrP antibody 3F4 and PrPSc-type specific antibodies T1 or T2. (d) The mean signal intensities of 3F4-, T1-, or T2-reactive PrPSc. To compare the amounts of the T1- or T2-reactive PrPSc among the mice, the signal intensities of the T1- or T2-reactive PrPSc were normalized by those of the 3F4-reactive PrPSc. The mean signal intensities of MM1-inoculated Ki-Hu129M/M were assigned as 100 /mm2 in each experiment using 3F4 or T1 antibody, and those of MV2K + C-inoculated Ki-Hu129V/V were assigned as 100 /mm2 in each experiment using T2 antibody. All experiments were repeated independently at least three times.