|
Antigens
|
Type
|
Clone / Name
|
Dilut.
|
Origin
|
Double stains with
|
|---|
|
α-smooth muscle actin
|
mouse monoclonal
|
1A4
|
1:500
|
Sigma
|
HNE
|
|
CD45 (leukocyte antigen)
|
mouse monoclonal
|
2B11 + PD7/26
|
1:100
|
DAKO
|
Oil-Red-O
|
|
CD68 (macrophage)
|
mouse monoclonal
|
PG-M1
|
1:100
|
DAKO
|
15-LOX
|
|
15-Lipoxygenase
|
rabbit polyclonal
|
CheY
|
1:1000
|
(18)
|
CD68
|
|
Native or oxidized LDL epitopes
| | | | | |
|
ApoB-100
|
mouse monoclonal
|
MB47
|
1:500
|
(10)
|
-
|
|
Minimally modified LDL
|
mouse monoclonal
|
Ox4E6
|
1:200-1000
|
(13)
|
1A4, YE
|
|
Copper oxidized LDL
|
guinea-pig polyclonal
|
YE
|
1:500
|
(14)
|
Ox4E6
|
|
Hydroxynonenal (OxLDL)
|
guinea-pig polyclonal
|
HNE
|
1:1000
|
(11)
|
1A4, MDA-2
|
|
Malondialdehyde (OxLDL)
|
mouse monoclonal
|
MDA-2
|
1:50
|
(11)
|
EO6
|
|
Natural anti-OxLDL IgM from ApoE mice
|
mouse monoclonal
|
EO6
|
1:500-1000
|
(12)
|
1A4, MDA2
|
- Sigma refers to Sigma-Aldrich Inc., St. Louis, MO. DAKO refers to DAKO A/S, Glostrup, Denmark. S-C refers to Santa-Cruz Biotechnology Inc, Santa-Cruz, CA, USA. For Anti-ApoB and oxidized LDL antibodies, the references were the antibodies were originally characterized are given in parentheses.
