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Table 3 Antibodies and dilutions used

From: Lipid accumulation, lipid oxidation, and low plasma levels of acquired antibodies against oxidized lipids associate with degeneration and rupture of the intracranial aneurysm wall

Antigens Type Clone / Name Dilut. Origin Double stains with
α-smooth muscle actin mouse monoclonal 1A4 1:500 Sigma HNE
CD45 (leukocyte antigen) mouse monoclonal 2B11 + PD7/26 1:100 DAKO Oil-Red-O
CD68 (macrophage) mouse monoclonal PG-M1 1:100 DAKO 15-LOX
15-Lipoxygenase rabbit polyclonal CheY 1:1000 (18) CD68
Native or oxidized LDL epitopes      
ApoB-100 mouse monoclonal MB47 1:500 (10) -
Minimally modified LDL mouse monoclonal Ox4E6 1:200-1000 (13) 1A4, YE
Copper oxidized LDL guinea-pig polyclonal YE 1:500 (14) Ox4E6
Hydroxynonenal (OxLDL) guinea-pig polyclonal HNE 1:1000 (11) 1A4, MDA-2
Malondialdehyde (OxLDL) mouse monoclonal MDA-2 1:50 (11) EO6
Natural anti-OxLDL IgM from ApoE mice mouse monoclonal EO6 1:500-1000 (12) 1A4, MDA2
  1. Sigma refers to Sigma-Aldrich Inc., St. Louis, MO. DAKO refers to DAKO A/S, Glostrup, Denmark. S-C refers to Santa-Cruz Biotechnology Inc, Santa-Cruz, CA, USA. For Anti-ApoB and oxidized LDL antibodies, the references were the antibodies were originally characterized are given in parentheses.