Astrocytic proteins are altered in lesion sites of at the late stage of chEAE and at the remitting stage of rrEAE. A and B, staining patterns of astrocytic proteins at the late stage of chEAE. C and D, staining pattern of astrocytic proteins at the remitting stage of rrEAE. E, Summary of the alteration of astrocytic proteins in chEAE. F, Summary of the alteration of astrocytic proteins in rrEAE. The “n” number of quantified images is provided for each bar. One-way ANOVA followed by Fisher’s test. Asterisk (*) shows significant difference from Control staining intensity for each antibody, p < 0.05. G, High magnification view of a lesion site at the late stage of chEAE. H, Confocal images of a lesion site at the remitting stage of rrEAE. Collapsed 2D image is on the top, and 3 cross sections are on the bottom. In (G), the crossbars are centered on an astrocyte with colocalizing AQP4 and GFAP. In (H), the crossbars emphasize the absence of AQP4 and GFAP in the lesion core. Scale bars, 500 μm in A-D, 50 μm in G,H.