Figure 9
Hepcidin visible in haem-rich deposit and red blood cells in AD brain. AD (SFG) human brain sections double-labeled with hepcidin and ferroportin by IFC and counterstained with DAPI. Limited hepcidin and FPN protein expression was seen throughout brain parenchyma with some autofluorescent puncta due to haem rich deposits ( HRD, a). Higher magnification showed both proteins in HRD, visible in small arterioles (b). A single labelled image showed RBCs with typical biconcave morphology were also positive for hepcidin (c). Scale bar: a 200 μm, b 50 μm, c 15 μm.