Figure 2

Hepcidin protein present in glial cells. In control brain sections from mid temporal gyrus (MTG) hepcidin expression was in astrocytes and co-localised with GFAP ( white arrow, a-d ). Hepcidin was visible in red blood cells close to blood vessels (e). Similarly in MTG in white matter tract hepcidin co-localised with myelin basic protein (MBP), suggesting that hepcidin protein is expressed in oligodendrocytes as well as in astrocytes (f-h). Scale bar panels a-c and e 30 μm, d 15 μm, f-h 20 μm. i. Hepcidin protein levels in the human superior frontal gyrus (SFG) of AD brains compared to controls. Densitometric measurements of corrected total cell fluorescence adjusted against background (n=20, p<0.0001). j. Ferroportin protein level in human SFG of AD brain compared to controls (n=20, p<0.0001) k. Hepcidin protein level in the dentate gyrus of APP-tg mice vs control (n= 20, p<0.0001). ***denotes p<0.0001.