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Table 1 The ingredients of qRT-QuIC preparation

From: Establishing quantitative real-time quaking-induced conversion (qRT-QuIC) for highly sensitive detection and quantification of PrPSc in prion-infected tissues

Component volume
10 x QuIC buffer 10 μl
(100 mM NaH2PO4, 100 mM Na2HPO4, 10 mM EDTA, 1.3 M NaCl, pH 6.9)
dd H2O 35 μl for standard curve, not for testing tissue extract
3 M NaCl 10 μl
rPrPsen (0.3 mg/ml) 34 μl (final 0.1 mg/ml)
1 mM thioflavin T 1 μl
seed 10 μl for standard curve, 45 μl for testing tissue extract
total volume of each RT-QuIC 100 μl