Figure 3From: Calreticulin and other components of endoplasmic reticulum stress in rat and human inflammatory demyelination Immunohistochemical staining of CHOP and XBP1 expression in spinal cord EAE lesions. CHOP and XBP1 were positively expressed in spinal cord EAE lesions (b &g, respectively) but were minimal or absent in IFA control tissue (a &f). At a higher magnification (h), clear positive/nuclear staining of cells with morphological ‘street-like’ features of oligodendrcoytes is present. A ventro-lateral white matter lesion dual-labelled using antibodies to SMI32, NeuN and CHOP shows a large number of CHOP-positive cells with features consistent with microglia/macrophages within the lesion centre (d). Co-localisation of CHOP in macrophage/microglia was confirmed (see ‘e’) by dual immunofluorescence (Iba1+/green, CHOP/red). Scale bars = 100 μm (d), 50 μm (a-c; e, f-g), 20 μm (h).Back to article page