Figure 2

CRT staining in spinal cord EAE lesions. There was little or no CRT expressed in IFA-injected spinal cords (a) compared to EAE lesions (b). At increased magnification, CRT staining was evident in a number of cell types, morphologically resembling astrocytes (c) and macrophages (d). CRT was expressed in Oil Red O deposits (f and digitally zoomed inset). ORO deposits colocalising with CRT were absent from IFA control spinal cord tissue (e). Fluorescent dual-labelled images (g-i) show CRT (red) expression in astrocytes (GFAP, green), as well as macrophage/microglia (Iba1⁺, green, k). Confocal analysis demonstrated punctate staining of CRT in microglia (j). Scale bars = 60 μm (g-i), 50 μm (a-b,e-f,j-k), 20 μm (c-d).