Figure 3

Reelin levels in the CSF. A) Anti-Reelin 142 immunoreactive signals on Western blots at low exposure time (40 seconds) to visualize the NR2 fragment. A’) Higher exposure time (18 minutes) of the same blot as A to visualize full-length (~460 kDa), NR6 (~ 310 kDa) and the additional band at ~ 60 kD. Images of immunoblots were cut in half to separate ND from AD for visual display. The short vertical lines at the bottom of the blot indicate joined bands for visual presentation. The HiMark Pre-Stained Standard was used as ladder. B) Semi-quantitative analysis of the levels of full-length Reelin and its proteolytic fragments in the CNS. Scatter plots representing relative optical densities expressed in arbitrary units (AU) of CSF samples obtained from ND and AD individuals. Lines represent the group median. Mann–Whitney U-test revealed no significant differences between groups.