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Table 2 Cases

From: Double-labelling immunohistochemistry for MGMT and a “cocktail” of non-tumourous elements is a reliable, quick and easy technique for inferring methylation status in glioblastomas and other primary brain tumours

case ID type LI SL MLPA MLPA 0-3 MLPA >0.25 DL
1 oligo 7.567 1 0.6275 2 1 1
2 oligo 13.700 1 0.675 2 1 1
3 oligo 12.600 1 0.8075 3 1 1
4 oligo 24.667 1 0.4725 1 1 1
5 oligo 17.100 1 0.8525 3 1 1
6 oligo 13.600 1 0.8025 3 1 1
7 oligo 19.567 1 0.6425 2 1 1
8 oligo 14.267 1 0.6925 2 1 1
9 oligo 8.800 1 0.6525 2 1 1
10 oligo 26.133 1 0.5925 2 1 *1
11 gbm 56.367 0 0.1125 0 0 0
12 gbm 19.167 1 0.8475 3 1 1
13 gbm 84.400 0 0.09 0 0 0
14 gbm 17.967 1 0.2625 1 1 *1
15 gbm 57.700 0 0.255 1 1 0
16 gbm 89.900 0 0.4075 1 1 0
17 gbm 72.267 0 0.2075 0 0 0
18 gbm 19.533 1 0.3525 1 1 *1
19 gbm 54.133 0 0.42 1 1 *1
20 gbm 53.900 0 0.1375 0 0 0
21 gbm 21.967 1 0.485 1 1 1
22 gbm 54.167 0 0.5475 2 1 1
23 gbm 33.000 1 0.085 0 0 0
24 gbm 76.300 0 0.2725 1 1 0
25 gbm 96.733 0 0.24 0 0 0
26 gbm 49.567 1 0.4275 1 1 1
27 gbm 43.967 1 0.1375 0 0 *1
28 gbm 40.533 1 0.1025 0 0 0
29 gbm 7.233 1 0.1275 0 0 1
30 gbm 39.067 1 0.5125 2 1 1
31 gbm 25.833 1 0.2075 0 0 *1
32 gbm 33.900 1 0.13 0 0 *1
33 gbm 28.000 1 0.365 1 1 0
34 gbm 45.000 1 0.275 1 1 1
35 gbm 84.100 0 0.205 0 0 0
36 gbm 85.100 0 0.2975 1 1 0
37 gbm 64.933 0 0.11 0 0 0
38 gbm 53.400 0 0.17 0 0 0
39 gbm 6.800 1 0.825 3 1 1
40 gbm 22.500 1 0.2425 0 0 0
  1. Case ID (identification) is the case number we assigned in this study. Tumour types were oligodendroglial tumours (oligo) and glioblastomas (gbm). The oligodendroglial tumours were oligodendroglioma WHO grade II (cases 4, 5 and 6), anaplastic oligodendroglioma WHO grade III (cases 1, 2, 3, 7 and 9), oligoastrocytoma WHO grade II (case 10) and anaplastic oligoastrocytoma WHO grade III (case 8). The glioblastomas were all glioblastoma WHO grade IV except cases 13 and 18 which were glioblastoma with oligodendroglioma component WHO grade IV. LI is the nuclear labelling index for single-labelled MGMT as determined by computerised image analysis. SL is the binarised score for the single-labelling (0 = LI > 50%, 1 = LI from 0 to 50%). MLPA is the MLPA ratio (please see text). MLPA 0–3 is the score for MLPA according to cut-offs for the MLPA ratio provided by Jeuken et al. 2007 [10] (0 = unmethylated, 1 = mildly methylated, 2 = moderately methylated and 3 = extensively methylated). MLPA > 0.25 is the binarised methylation score for MLPA (0 = unmethylated, 1 =methylated). DL is the binarised score for double labelling (0 = mainly MGMT-positive in tumour cells, 1 = mainly MGMT-negative in tumour cells). The asterisked cases were initially assigned a score of 1 in the tripartite system (please see text for explanation).